The role of oxidative stress, inflammation and acetaminophen exposure from birth to early childhood in the induction of autism William Parker, Chi Dang Hornik, Staci Bilbo, Zoie E. Holzknecht, Lauren Gentry, The gamma glutamyl pathway after glutathione depletion due to Download Scientific Diagram Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.